Shelley L. Berger, Ph.D.

Daniel S. Och University Professor; Director, Epigenetics Institute; Penn Integrated Knowledge Professor (PIK); Co-leader, Tumor Biology Program, Abramson Cancer Center

University of Pennsylvania
The Perelman School of Medicine
Department of Cell and Developmental Biology; Biology; Genetics
9-125 Smilow Center for Translational Research
3400 Civic Center Blvd
Philadelphia, PA 19104
Office: 215-746-3106

Lab: 215-746-8223

1.  Identification of transcriptional adaptors/coactivators Gcn5/Ada2/Ada3 and discovery of novel histone modifications and mechanisms in transcription and sperm genome opening.
We discovered transcriptional “adaptors”, which we showed associate with DNA binding activators, a groundbreaking new model for transcriptional activation, to reveal how histone enzymatic modifiers are recruited to genes. We revealed the importance of adaptor Gcn5 acetyltransferase activity in transcriptional activation (1998), to unify understanding of transcription and chromatin regulation. We discovered numerous novel histone modifications, modification cross-talk, and sequential histone modifications in transcription, including histone phosphorylation/acetylation (2001) and ubiquitylation/deubiquitylation. In 2017, we discovered that enhancer RNAs bind directly to CBP, the key metazoan acetyltransferase, to stimulate HAT activity in vitro and at enhancers and promoters in vivo.  We recently showed (2019) that Gcn5 provides key histone acetylation to broadly open the mouse genome during spermatogenesis for broad chromatin restructuring.

  • Wang L, Liu L. and Berger SL. (1998) Critical residues for histone acetylation by GCN5, functioning in Ada and SAGA complexes, are also required for transcriptional function in vivo. Genes & Development 12: 640-653. PMCID: PMC316586
  • Lo W-S, Duggan L, Belotserkovskya R, Emre T, Lane W, Shiekhattar R, and Berger SL.  (2001) Snf1 is a histone kinase which works in concert with the histone acetyltransferase Gcn5 to regulate transcription.  Science 293:1142-6. PMID:11498592
  • Bose DA, Donahue G, Reinberg D, Shiekhattar R, Bonasio R, Berger SL. (2017)  RNA binding to CBP stimulates histone acetylation and transcription. Cell 168,135-149. PMCID: PMC5325706.
  • Luense LJ, Donahue G, Lin-Shiao E….Bartolomei M, Berger SL. (2019) Gcn5-mediated histone acetylation governs nucleosome dynamics in spermiogenesis. Developmental Cell 51:745-758.

2. Discovery of chromatin mechanisms controlling aging and senescence in yeast and mammals.
Our work uncovered chromatin changes involved in aging and cellular senescence, indicating broad dysregulation of the epigenome. These include pioneering studies demonstrating that histone acetylation drives aging in yeast (2009) and disruption of the nuclear lamina with its associated chromatin domains in mammals.  We showed these disruptions trigger both homeostatic genomic protection and cellular damage, and discovery of nuclear autophagy pathways in senescence leading to inflammation in aging and cancer (2015, 2017, 2019, 2020). Our findings suggest potential epigenetic therapeutics to ameliorate age-associated disease.

  • Dang W…Kaeberlein M, Kennedy BK, and Berger SL. (2009) Histone H4 lysine-16 acetylation regulates cellular lifespan. Nature 459:802-7. PMCID: PMC2702157.
  • Dou Z…^Adams PD, and ^Berger SL. (2015) Autophagy mediates degradation of nuclear lamina. Nature 527:105-9. PMCID: PMC4824414.   (2017) Cytoplasmic chromatin triggers inflammation in senescence and cancer. Nature, 550:402-406.  PMID:28976970.
  • Sen P, Lan Y…Adams PD, Schultz DC, Berger SL. (2019) Histone acetyltransferase p300 induces de novo super-enhancers to drive cellular senescence. Molecular Cell, 73:684-698. PMID:30773298.
  • Xu C, Wang L…Adams PA, Ott M, Tong W, Johansen T, Dou Z^, and Berger SL^.  (2020)  SIRT1 is downregulated by autophagy in senescence and aging.  Nature Cell Biology, Oct 9..

3. Demonstration of chromatin mechanisms controlling memory and behavior. 
Our studies in mouse brain and memory show a pivotal role of the metabolic enzyme, ACSS2, in fueling “on-site” acetyl-CoA generation on chromatin for neuronal histone acetylation and gene expression in normal memory and in alcohol-fueled addiction memory (2017/19). Our work in human Alzheimer’s disease reveals that the cognitively normal aging brain is epigenetically protected compared to the AD brain (2018/20). In other research on brain, we pioneered investigation of eusocial ant caste-specific behavior for organismal-level chromatin regulation and epigenetics, owing to the remarkable fact that female ants of distinct social castes (such as queen, soldier, and forager) share an identical genome. We sequenced the first ant genomes and then profiled the first histone modification epigenomes (2013). Groundbreaking results indicate a critical role of histone modifications in altering ant brain function to instruct complex social behavior; we identified a “window”, early after hatching, to behavioral reprogramming via epigenetic manipulation (2016).  We pioneered Crisper genetics in ants (2017).

  • Simola DF…^Reinberg D, ^Liebig J, ^Berger SL.  (2016)  Epigenetic (re)programming of caste-specific behavior in the ant C. floridanus. Science 351:aac6633. PMID: 26722000, PMCID: PMC5057185.
  • Mews, P, Donahue G… Berger SL. (2017) Acetyl-CoA metabolism by ACSS2 regulates neuronal histone acetylation and hippocampal memory. Nature 546,381-386. PMCID: PMC5505514.  Mews P, ^Egervari G…Garcia, B, ^Berger SL. (2019) Alcohol metabolism contributes to brain histone acetylation. Nature 574: 717-721.
  • Glastad K, Graham RJ, Ju L, Rossler J, Brady CM, and Berger SL (2019) Epigenetic regulator CoRest controls social behavior in ants.  Molecular Cell 77:338-351.
  • Nativio R, Lan Y…Garcia BA, Trojanowski JQ, Bonini NM^, Berger SL^.  (2020) An integrated multi-omics approach identifies epigenetic drivers associated with Alzheimer’s disease.  Nature Genetics 52:1024-1035.

4. Discovery of tumor suppressor p53 factor and histone modifications and their mechanisms including activating p53 acetylation, repressive p53 methylation, and novel chromatin pathways in p53-mediated transcriptional activation.
Our work revealed new enzyme modifiers and post-translational modifications of p53 (including acetylation, methylation, and demethylation, 2006/7/10)) regulating p53 activity. Our findings spurred broad efforts to discover novel transcription factor modifications. We showed p53 methylation is generally repressive to its function, and showed repressive p53 methylation occurring in certain cancers bearing high levels of wild type p53. We discovered novel epigenetic pathways used by wild type and mutant p53 in regulating chromatin structure/function in normal and cancer cells, such as gain-of-function p53 mutants driving transcriptional activating and growth promoting histone modifications (2015).  We showed that p53 and p63 (2019) establish new enhancers during stress and development.

  • Huang J…Jenuwein T, and Berger SL. (2006) Repression of p53 activity by Smyd2-mediated methylation.  Nature 444:629-32. PMID:17108971.
  • Bungard D…Thompson CB, Jones RG and Berger SL. (2010) Signaling kinase AMPK activates stress-promoted transcription via histone H2B phosphorylation. Science 329: 1201-5. PMCID: PMC3922052.
  • Zhu J, Sammons MA, Donahue G, Dou Z…Arrowsmith CH, and Berger SL. (2015) Gain-of-function p53 mutants co-opt epigenetic pathways to drive cancer growth. Nature 525:206-11. PMCID: PMC4568559
  • Lin-Shiao E, Lan Y…Sammons M, Ludwig K, and Berger SL. (2019) p63 establishes epithelial enhancers at critical craniofacial development genes. Science Advances, May 1; 5:eaaw0946.

5. Investigation of epigenetic mechanisms affecting cancer immunotherapy.
We established collaborations with Carl June (pioneered CAR T cell therapy in cancer) and John Wherry (discovered key aspects of T cell exhaustion).  We investigate epigenetic regulation in patient response to immunotherapy, and controlling T cell exhaustion.

  • Pauken KE…Berger SL, and Wherry EJ.  (2016) Epigenetic stability of exhausted T cells limits durability of reinvigoration by PD-1 blockade. Science 354,1160-1165.
  •  Fraietta JA…Berger SL, Bushman FD, June CH, and Melenhorst JJ. (2018) Disruption of TET2 promotes the therapeutic efficacy of CD19-targeted T-cells. Nature 555, 307-312.
  • Khan O…Berger SL, and Wherry EJ.  (2019) TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion.  Nature 571, 211-218.

Research Interest

Our lab focuses on mechanisms that regulate gene expression with a special emphasis on how the DNA-packaging structure of chromatin is manipulated during genomic processes. Our findings inform the study of cancer and other diseases, and ultimately drug discovery.

Lab Members