Shared Resources

PSOM Biobank

Penn Medicine BioBank (PMBB)
Perelman School of Medicine at the University of Pennsylvania (PSOM)
The Penn Medicine BioBank (PMBB) is a unique community-driven resource that provides controlled access to biospecimens, and genetic and clinical data from over 45,000 PMBB participants.
Our combination of biospecimens (DNA, blood cells, serum, plasma and various residual tissues), access to Electronic Health Records (EHRs), and genotyping and whole exome sequencing data, makes PMBB a valuable partner for diverse studies ranging from understanding human genetic variation in health and disease, biomarker-discovery opportunities to enhancing recruitment for clinical studies that require participation of subjects with very specific clinical profiles.
By establishing and maintaining a high quality IRB-approved biobank, PSOM and PMBB contribute to fueling cutting-edge basic and translational biomedical research.
Please visit www.PennMedicineBioBank.org for updates about PMBB and PSOM-supported biobanking initiatives, or schedule a call or meeting with us at calendly.com/pennmedbiobank.
Contact:
Cristian A. Perez PhD
Director of Alliances Penn Medicine BioBank
criperez@pennmedicine.upenn.edu
Cristian@PennMedicineBioBank.org
215-746-0358
Schedule a PMBB Team call/meeting: https://calendly.com/pennmedbiobank
http://www.PennMedicineBioBank.org

Protocols

We are soliciting protocols, from anyone in the epigenetics community that may be useful to the community at large.  They will be posted here to be seen by a large audience and to facilitate both research and collaboration. Please contact andes@mail.med.upenn.edu if you would like to share!
From Shelley Berger’s lab:

From Arjun Raj’s lab:

Small Sample ChIP-seq Method:

Obtaining genome-wide information from small tissues or from few cells has been a technical challenge largely due to the inefficiency of several enzymatic reactions needed to prepare samples for high-throughput sequencing (ChIP-seq or RNA-seq). Recently, however, two advances have enabled robust sequencing of samples with little starting material. First, protocols for sequence library preparation have been optimized, allowing library preparation from as little as 500pg of dsDNA. Second, strategies pioneered for quantitation of RNA from single cells, such as linear amplification using the bacteriophage T7 RNA polymerase, have been adapted for ChIP. This method provides up to 100-fold amplification of material with one round of in vitro transcription. For example, a single haploid mouse cell contains ~2pg of dsDNA and approximately the same amount of RNA. Thus, a single round of linear amplification will yield ~200pg of dsDNA, theoretically allowing ChIP-seq for a sample pooling as few as 10 cells.