Resources

Graphical Interpretation and Analysis of Proteins and their Ontologies (Gia Pronto) data analysis and visualization software

JoVE video of protocol for histone isolation and preparation for Bottom Up MS

Protocol for nuclei isolation and acid extraction of histones

Protocol for propionylation of histones

Our EpiProfile software (EpiProfile 2.0: A Computational Platform for Processing Epi-Proteomics Mass Spectrometry Data. Yuan ZF, Sidoli S, Marchione DM, Simithy J, Janssen KA, Szurgot MR, Garcia BA. J Proteome Res. 2018 Jul 6;17(7):2533-2541) to quantify histone PTMs is freely available upon request (please send an email to zuoyuan@mail.med.upenn.edu)

To discuss obtaining our heavy isotope labeled synthetic histone peptide library (Lin et al. Mol Cell Proteomics. 2014, 13(9), 2450-2466), please contact Ben Garcia (bgarci@mail.med.upenn.edu)

Quick guide protocol for Bottom Up Proteomic Analysis

Histone Proteomics Formal Protocols (Karch et al. Methods in Enzymology, 574, 3-29, 2016)